Western Blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Marazziti, Di Pietro, Mandillo, Golini, Matteoni, Tocchini-Valentini: "Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, DeltaFosB expression, and conditioned place preference to amphetamine and cocaine." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 2071-81, (2011) (PubMed).
Maiya, Zhou, Norris, Kreek, Strickland: "Tissue plasminogen activator modulates the cellular and behavioral response to cocaine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 6, pp. 1983-8, (2009) (PubMed).
Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, (2008) (PubMed).
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B