Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This antibody is specific for the IFNAR1 protein phosphorylated at Ser535,539. Note: The Molecular Weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells, the mature form is ~110-130k.
Cross-Reactivity (Details)
Species reactivity (expected):Bovine, Canine, Human, Mouse, Sheep and non-Human Primates. Species reactivity (tested):Rat.
Purification
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser535,539 of Human IFNAR1.
IFNAR1
Reactivity: Human
WB
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Western blot: 1/1000. Immunohistochemistry: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Bhattacharya, HuangFu, Liu, Veeranki, Baker, Koumenis, Diehl, Fuchs: "Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2318-25, (2010) (PubMed).
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).Synonyms: CRF2-1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, IFN-R-1, IFN-alpha Receptor alpha, IFN-alpha/beta receptor 1, Interferon-alpha/beta receptor alpha chain, Type I interferon receptor 1