Cortactin antibody (N-Term)

Catalog No. ABIN374140

Product Details anti-Cortactin Antibody

Target: Cortactin (CTTN)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: WKASAGHAVS IA-C

Specificity: This antibody detects Cortactin at N-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_005222.2, NP_612632.1 Genename: CTTN

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx.80 kDa band observed in Mouse and Rat Brainlysates (calculated MW of 61.0 kDa according to Human NP_005222.2 and of 61.0 kDaaccording to Mouse NP_031829.2). The observed molecular weight corresponds to earlierfindings in literature with different antibodies (Paradis et al, J Cell Sci.2008 Jun 15,121(Pt12): 1965-72, PMID 18495841).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin (CTTN Products)

Synonyms: EMS1 antibody, 1110020L01Rik antibody, Ems1 antibody, Cttnb antibody, CG3637 antibody, Dmel\CG3637 antibody, cortactin antibody, cttn antibody, CTTN1 antibody, P85.25 antibody, Cttn antibody, ems1 antibody, CTTN antibody, cortactin antibody, CG3637 gene product from transcript CG3637-RD antibody, cortactin S homeolog antibody, cortactin L homeolog antibody, src substrate cortactin antibody, Src substrate cortactin antibody, CTTN antibody, Cttn antibody, Cortactin antibody, cttn.S antibody, cttn.L antibody, cttn antibody, CpipJ_CPIJ006351 antibody, Bm1_57220 antibody, LOC100533277 antibody

Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1

Gene ID: 2017

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: MAPK Signaling