Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen
Peptide from C Terminus of the protein sequence according to NP_005638.1, NP_001132938.1, NP_001132939.1, NP_001132940.1, NP_078941.2. Genename: TBL1X / TBL1XR1
TBL1X
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Peptide ELISA: Limit dilution 1: 4000. Western blot: 3-5 μg/mL. Approx 65-70 kDa band seen in Human Brain lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.