Paired Box 3 antibody (N-Term)
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- Target See all Paired Box 3 (PAX3) Antibodies
- Paired Box 3 (PAX3)
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Cow
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Paired Box 3 antibody is un-conjugated
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Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Sequence
- TTLAGAVPRM M-C
- Specificity
- This antibody detects PAX3 at N-term. This antibody is expected to recognise all 7 reported human isoforms (as represented by NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1 and NP_852126.1).
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Rat and Cow.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Peptide from the N Terminus of the protein sequence according to NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1. Genename: PAX3
- Top Product
- Discover our top product PAX3 Primary Antibody
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- Application Notes
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Peptide ELISA: Antibody Limit Dilution: 1/64000. Western blot: Preliminary experiments gave an approx 30-35 kDa band in Human Kidneylysates at 1 μg/mL. Immunohistochemistry: 3 μg/mL. In paraffin embedded Human Oesophagus showsnuclear staining of cells in the Squamous Epithelium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
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- Target
- Paired Box 3 (PAX3)
- Alternative Name
- PAX3 (PAX3 Products)
- Synonyms
- PAX3 antibody, cdhs antibody, hup2 antibody, pax-3 antibody, ws1 antibody, xpax3 antibody, Pax3 antibody, pax3 antibody, pax3b antibody, xPax3-B antibody, CDHS antibody, HUP2 antibody, WS1 antibody, WS3 antibody, Pax-3 antibody, Sp antibody, splotch antibody, zgc:92547 antibody, paired box 3 antibody, paired box 3 L homeolog antibody, paired box 3a antibody, PAX3 antibody, pax3 antibody, pax3.L antibody, Pax3 antibody, pax3a antibody
- Background
- This protein is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2,13)(q35,q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.Synonyms: HUP2, Paired box protein Pax-3, Pax-3
- Gene ID
- 5077
- NCBI Accession
- NP_000429
- UniProt
- P23760
- Pathways
- Sensory Perception of Sound, Tube Formation
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