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MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term)

Details for Product anti-MCL1 Antibody No. ABIN374436, Supplier: Log in to see
Antigen
  • AW556805
  • Mcl-1
  • BCL2L3
  • EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • TM
  • bcl2-L-3
  • mcl1/EAT
  • mcl-1
Alternatives
anti-Human MCL-1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term, Isoform 1
31
23
22
15
15
13
13
13
11
10
9
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
291
101
67
8
8
5
4
3
2
1
1
1
Host
Goat
206
76
17
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
11
9
9
6
6
5
3
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
245
118
103
58
55
31
26
22
21
8
7
6
1
Supplier
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Immunogen Peptide from the C Terminus of the protein sequence according to NP_068779.1
Sequence C-AGVGAGLAYL IR
Specificity This antibody is expected to recognise isoform 1 (NP_068779.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog) and Feline (Cat).
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name MCL1 (MCL1 Antibody Abstract)
Background Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004
Gene ID 4170
NCBI Accession NP_068779
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling
Application Notes Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of Human Lung and cellline K562 (calculated MW of 37.5 kDa according to NP_068779.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term) (ABIN374436) anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (C-Term), (Isoform 1) antibody
Background publications Opferman, Letai, Beard et al.: "Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1." in: Nature, Vol. 426, Issue 6967, pp. 671-6, 2003 (PubMed).