Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Sequence
C-EQYQKTSLAQ EKEK
Specificity
This antibody reacts to CD249. Approx 170 kDa band observed in Human Brain, Kidney and Testis lysates (calculated MW of 109 kDa according to NP_001968.2).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine. Species reactivity (tested):Human.
Purification
Affinity chromatography
Immunogen
Peptide with sequence from the internal region of the protein
ENPEP
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: 1/64000. Western Blot: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: 3 - 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
ENPEP
(Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))
BP1, also known as Ly51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide linked chains of 140 kDa. Originally identified on the surface of pre B cell lymphomas the cDNA encoding BP1 has been cloned and found to represent aminopeptidase A. It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity. Subsets of normal bone marrow pre B and B cells express low levels of BP1, which is rapidly upregulated on pre B cells in the presence of IL7.Synonyms: Aminopeptidase A, Differentiation antigen gp160, EAP, ENPEP