GRIN2B antibody (Internal Region)

Catalog No. ABIN374702

Product Details anti-GRIN2B Antibody

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-KDAHEKDDFH HLS

Specificity: This antibody is specific to NMDAR2B / GRIN2B.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Rat and Dog (Canine).
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000825.1

Application Details

Application Notes: Peptide ELISA: Antibody detection limit dilution 1/16,000. Western Blot: (2-4 μg/mL). A approx. 170 kDa band is observed in Rat Brain lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: NMDAR2B (GRIN2B Products)

Synonyms: NMDAR2B antibody, GluN2B antibody, MRD6 antibody, NR2B antibody, hNR3 antibody, nmdar2b antibody, nr2b antibody, AW490526 antibody, Nmdar2b antibody, glutamate ionotropic receptor NMDA type subunit 2B antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog antibody, glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody, GRIN2B antibody, Grin2b antibody, grin2b.L antibody

Background: NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain types of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease, epilepsy and ischemic neuronal cell death.Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B

Gene ID: 2904

NCBI Accession: NP_000825

UniProt: Q13224

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity