NCF4 antibody (Internal Region)

Catalog No. ABIN374770

This Goat Polyclonal antibody specifically detects NCF4 in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Dog.

Quick Overview for NCF4 antibody (Internal Region) (ABIN374770)

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-NCF4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to NCF4 / P40PHOX.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in lysates of cell line Daudi (calculated MW of39.8 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). Intransfected HEK293 transiently expressing P40PHOX a band of approx.48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Tonsil shows staining of thecytoplasm in some cells. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: NCF-4

Background: NCF4 is a cytosolic oxidase associated protein. It is a component of the nicotinamide adenine dinucleotide phosphate oxidase, which mediates down regulation of NADPH oxidase through interactions with its SH3 domain. NCF4 is associated with p67 phox but is absent in patients with chronic granulomatous disease who lack p67 phox.Synonyms: Neutrophil NADPH oxidase factor 4, Neutrophil cytosol factor 4, SH3 and PX domain-containing protein 4, SH3PXD4, p40-phox

Gene ID: 4689

NCBI Accession: NP_000622

UniProt: Q15080