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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) antibody Primary Antibody

NCF4 Reactivity: Human IHC, ELISA, WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN190755
$481.84
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100 μg
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  • Target
    Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
    Binding Specificity
    Internal Region
    Reactivity
    Human
    Host
    Goat
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Purpose
    NCF4 / P40PHOX
    Sequence
    KDFPEEDDPT N
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
    Predicted Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.
    Isotype
    IgG
  • Application Notes

    DS WB Results: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

    DS IHC Results: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 2.5 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
    Alternative Name
    NCF4 (NCF4 Antibody Abstract)
    Synonyms
    p40phox, AI451400, NCF, P40PHOX, SH3PXD4, neutrophil cytosolic factor 4, ncf4, Ncf4, NCF4
    Background
    NCF4, P40PHOX, neutrophil cytosolic factor 4, 40 kDa , CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)
    Gene ID
    4689
    NCBI Accession
    NP_000622, NP_038202
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