GPX3 antibody (Internal Region)

Catalog No. ABIN374849

Product Details anti-GPX3 Antibody

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GPX3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-NQFGKQEPGE NSE

Specificity: This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Canine.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002075.2.

Application Details

Application Notes: Peptide ELISA: Limit dilution: 1/1000. Western Blot: 1-3 μg/mL. Approx 26 kDa band observed in Mouse Kidney and Liver lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GPX3

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Alternative Name: Glutathione Peroxidase 3 / GPX3 (GPX3 Products)

Synonyms: GPX3 antibody, DKFZp469D1932 antibody, AA960521 antibody, EGPx antibody, GPx antibody, GSHPx-3 antibody, GSHPx-P antibody, GPx-3 antibody, GPx-P antibody, Gpxp antibody, glutathione peroxidase 3 antibody, gpx3 antibody, GPX3 antibody, Gpx3 antibody

Background: Glutathione peroxidases(Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organichydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPxfamily to be discovered. GPx2, also known as gastrointestinal GPx(GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract.Extracellular plasma GPx(pGPxor GPx3) is mainly expressed by the kidney from where it is released into the blood. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxylipids in low density lipoprotein orthymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site.Synonyms: Extracellular glutathione peroxidase, GPX-3, GPXP, GPx-3, GPx-P, GSHPXP, GSHPx-3, GSHPx-P, Glutathione peroxidase-3, Plasma glutathione peroxidase

Gene ID: 2878

NCBI Accession: NP_002075

UniProt: P22352

Pathways: Thyroid Hormone Synthesis