SIGLEC8 antibody (Internal Region)

Catalog No. ABIN375053

Product Details anti-SIGLEC8 Antibody

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to SIGLEC8.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-SYKSQLNYKTKQ, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-SIGLEC8 antibody (ABIN375053)

Yokoi, Choi, Hubbard, Lee, Canning, Lee, Ryu, von Gunten, Bickel, Hudson, Macglashan, Bochner: "Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement." in: The Journal of allergy and clinical immunology, Vol. 121, Issue 2, pp. 499-505.e1, (2008) (PubMed).

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: CD329 / SIGLEC8 (SIGLEC8 Products)

Synonyms: SAF2 antibody, SIGLEC-8 antibody, SIGLEC8L antibody, SIGLEC8 antibody, sialic acid binding Ig like lectin 8 antibody, sialic acid-binding Ig-like lectin 8 antibody, SIGLEC8 antibody, LOC506364 antibody

Background: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.Synonyms: CDw329, SAF2, Sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2, Siglec-8

Gene ID: 27181, 9606

UniProt: Q9NYZ4