SIGLEC8 antibody (Internal Region)

Catalog No. ABIN308467

This Goat Polyclonal antibody specifically detects SIGLEC8 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SIGLEC8 antibody (Internal Region) (ABIN308467)

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SIGLEC8 Antibody

Binding Specificity: Internal Region

Purpose: SIGLEC8

Sequence: SYKSQLNYKT KQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SYKSQLNYKTKQ, from the internal region of the protein sequence according to NP_055257.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 45 kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0 kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 J

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: SIGLEC8

Background: SIGLEC8, sialic acid binding Ig-like lectin 8, MGC59785, SAF2, SIGLEC-8, SIGLEC8L

Gene ID: 27181

NCBI Accession: NP_055257