GCKR antibody (N-Term)

Catalog No. ABIN375154

This Goat Polyclonal antibody specifically detects GCKR in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GCKR antibody (N-Term) (ABIN375154)

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCKR Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to GCKR.

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse, Rat.

Purification: Affinity chromatography

Immunogen: Peptide with sequence KRFQHVIETPEPGK -C, from the N-Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GCKR

Background: GCKR is a subfamily member of the SIS (Sugar ISomerase) family of proteins. It is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).Synonyms: GCKR, Glucokinase regulator, Glucokinase regulatory protein

Gene ID: 2646, 9606

UniProt: Q14397

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus