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UBA3 antibody (Ubiquitin-Like Modifier Activating Enzyme 3) (AA 415-445)

Details for Product anti-UBA3 Antibody No. ABIN387909, Supplier: Log in to see
Antigen
  • UBE1C
  • hUBA3
  • A830034N06Rik
  • AI256736
  • AI848246
  • AW546539
  • Ube1c
  • ube1c
  • wu:fb75e04
  • wu:fc37b11
  • zgc:55528
Epitope
AA 415-445, C-Term
19
4
1
1
1
Reactivity
Human, Mouse (Murine)
29
9
5
2
2
2
2
1
1
Host
Rabbit
29
Clonality (Clone)
Polyclonal ()
Conjugate
This UBA3 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
27
18
17
3
1
Supplier
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Immunogen This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 AA from the C-terminal region of human UBE1C.
Clone RB2803
Isotype Ig
Specificity This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423~452 amino acids from the C-terminal region of human UBE1C.
Predicted Reactivity Rat (Rattus),Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name UBE1C (UBA3 Antibody Abstract)
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Synonyms: NEDD8-activating enzyme E1 catalytic subunit, Ubiquitin-activating enzyme 3, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, UBA3
Molecular Weight 51719 DA
Gene ID 9039
UniProt Q8TBC4
Research Area Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-UBA3 antibody (Ubiquitin-Like Modifier Activating Enzyme 3) (AA 415-445) (ABIN387909) The anti-UBE1 C-term Pab (ABIN387909) is used in Western blot to detect UBE1 in mouse...
Immunohistochemistry (IHC) image for anti-UBA3 antibody (Ubiquitin-Like Modifier Activating Enzyme 3) (AA 415-445) (ABIN387909) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-UBA3 antibody (Ubiquitin-Like Modifier Activating Enzyme 3) (AA 415-445) (ABIN387909) UBE1C Antibody (S430) (ABIN387909) western blot analysis in HL-60 cell line lysates (...
Background publications Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003 (PubMed).

Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003 (PubMed).

Gubin, Njoroge, Bouffard et al.: "Gene expression in proliferating human erythroid cells." in: Genomics, Vol. 59, Issue 2, pp. 168-77, 1999 (PubMed).

Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).

Osaka, Kawasaki, Aida et al.: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998 (PubMed).