Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term), (AA 1-32) antibody

Details for Product No. ABIN388037
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Antigen
Synonyms CG10679, Dmel\\CG10679, NEDD8, dNEDD8, nedd8, ubiquitin, si:zc14a17.5, GB17785, DDBDRAFT_0218116, DDBDRAFT_0238041, DDB_0218116, DDB_0238041, NEDD-8, Rub1
Epitope
N-Term, AA 1-32
(21), (3), (3), (1), (1), (1), (1)
Reactivity
Human
(68), (38), (36), (12), (12), (1), (1)
Host
Rabbit
(55), (11), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(61), (35), (32), (14), (10), (10), (7), (7), (3), (3), (2), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN388037
291.50 $
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Immunogen This NEDD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 AA from the N-terminal region of human NEDD8.
Clone RB0639
Isotype Ig
Specificity This NEDD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human NEDD8.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NEDD8
Background NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Synonyms: Ubiquitin-like protein NEDD8, Neddylin
Molecular Weight 9072 DA
Gene ID 4738
UniProt Q15843
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term), (AA 1-32) antibody Western blot analysis of NEDD8 polyclonal antibody (ABIN388037) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term), (AA 1-32) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Fan, Bigsby, Nephew: "The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast cancer cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 3, pp. 356-65, 2003 (PubMed).

Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003 (PubMed).

Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003 (PubMed).

Wu, Yamoah, Dolios et al.: "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28882-91, 2003 (PubMed).

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