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SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731), (C-Term) antibody

Details for Product No. ABIN388831, Supplier: Log in to see
Antigen
  • SMURF2
  • 2810411E22Rik
  • AI558114
  • AI649275
  • RGD1310067
  • wu:fb82a11
  • wu:fc41c02
Epitope
AA 702-731, C-Term
25
16
14
13
6
2
2
1
1
Reactivity
Human
65
21
15
Host
Rabbit
57
6
2
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
31
29
11
10
10
7
2
Supplier
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Immunogen This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 AA from the C-terminal region of human SMURF2.
Clone RB4107-4108
Isotype Ig
Specificity This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714~743 amino acids from the C-terminal region of human SMURF2.
Predicted Reactivity Mouse (Murine),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SMURF2 (SMURF2 Antibody Abstract)
Background SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Synonyms: E3 ubiquitin-protein ligase SMURF2, Smad ubiquitination regulatory factor 2, Smad-specific E3 ubiquitin ligase 2,
Molecular Weight 86113 DA
Gene ID 64750
UniProt Q9HAU4
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731), (C-Term) antibody (ABIN388831) Western blot analysis of anti-SMURF2 Pab (ABIN388831) in 293 cell line lysate (35 µg/...
Immunohistochemistry (IHC) image for anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731), (C-Term) antibody (ABIN388831) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Immunohistochemistry (IHC) image for anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 702-731), (C-Term) antibody (ABIN388831) Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Ki...
Product cited in: Schwamborn, Khazaei, Püschel: "The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 35259-68, 2007 (PubMed).

Background publications Tajima, Goto, Yoshida et al.: "Chromosomal region maintenance 1 (CRM1)-dependent nuclear export of Smad ubiquitin regulatory factor 1 (Smurf1) is essential for negative regulation of transforming growth factor-beta signaling by Smad7." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10716-21, 2003 (PubMed).

Suzuki, Murakami, Fukuchi et al.: "Smurf1 regulates the inhibitory activity of Smad7 by targeting Smad7 to the plasma membrane." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39919-25, 2002 (PubMed).

Ebisawa, Fukuchi, Murakami et al.: "Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12477-80, 2001 (PubMed).

Zhu, Kavsak, Abdollah et al.: "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." in: Nature, Vol. 400, Issue 6745, pp. 687-93, 1999 (PubMed).