This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This SPTLC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-437 amino acids from the C-terminal region of human SPTLC1.
SPTLC1
Reactivity: Human
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Wei, Yerokun, Leipelt, Haynes, Radhakrishna, Momin, Kelly, Park, Wang, Carton, Uhlinger, Merrill: "Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphology." in: Biochimica et biophysica acta, Vol. 1791, Issue 8, pp. 746-56, (2009) (PubMed).
Medler, Petrusca, Lee, Hubbard, Berdyshev, Skirball, Kamocki, Schuchman, Tuder, Petrache: "Apoptotic sphingolipid signaling by ceramides in lung endothelial cells." in: American journal of respiratory cell and molecular biology, Vol. 38, Issue 6, pp. 639-46, (2008) (PubMed).
Target
SPTLC1
(serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.