RFC5 antibody (AA 170-200)
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- Target See all RFC5 Antibodies
- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
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Binding Specificity
- AA 170-200
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RFC5 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS)
- Purification
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Immunogen
- This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human RFC5.
- Clone
- RB17319
- Isotype
- Ig Fraction
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- Application Notes
- WB: 1:1000. WB: 1:1000. WB: 1:1000. FC: 1:10~50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
- Expiry Date
- 6 months
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- Target
- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
- Alternative Name
- RFC5 (RFC5 Products)
- Synonyms
- zgc:110313 antibody, 2610020K06Rik antibody, 2610209F07Rik antibody, 36.5kDa antibody, 36kDa antibody, Recc5 antibody, RFC36 antibody, replication factor C (activator 1) 5 antibody, replication factor C subunit 5 antibody, replication factor C subunit 5 L homeolog antibody, rfc5 antibody, Rfc5 antibody, RFC5 antibody, rfc5.L antibody
- Background
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
- Molecular Weight
- 38497
- Gene ID
- 5985
- NCBI Accession
- NP_001123584, NP_031396, NP_853556
- UniProt
- P40937
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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