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CDC25A antibody (Cell Division Cycle 25 Homolog A (S. Pombe)) (pThr506)

Details for Product anti-CDC25A Antibody No. ABIN389532, Supplier: Log in to see
Antigen
  • cdc25A
  • CDC25A2
  • cdc25a2
  • D9Ertd393e
  • sb:cb317
Alternatives
anti-Human CDC25A antibody for Immunofluorescence
Epitope
pThr506
40
39
33
26
25
23
18
17
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16
14
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13
10
9
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6
6
6
6
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3
3
3
3
3
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2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
465
161
158
6
2
2
2
1
1
1
Host
Rabbit
435
33
Clonality (Clone)
Polyclonal ()
Conjugate
This CDC25A antibody is un-conjugated
22
22
22
18
18
18
8
4
4
4
4
4
4
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4
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
373
190
153
52
50
45
19
11
9
3
3
2
1
1
Supplier
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Immunogen This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.
Clone RB7856
Isotype Ig
Specificity This Phospho-CDC25A-T507 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human Cdc25A.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CDC25A (CDC25A Antibody Abstract)
Background CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms: M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A,
UniProt P30304
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure
Pathways Cell Division Cycle
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-CDC25A antibody (Cell Division Cycle 25 Homolog A (S. Pombe)) (pThr506) (ABIN389532) The anti-Phospho-CDC25A-T506 Pab (ABIN389532) is used in Western blot to detect Phosp...
Immunohistochemistry (IHC) image for anti-CDC25A antibody (Cell Division Cycle 25 Homolog A (S. Pombe)) (pThr506) (ABIN389532) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Chou, Yen, Lee, Chen: "Pro-apoptotic role of Cdc25A: activation of cyclin B1/Cdc2 by the Cdc25A C-terminal domain." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17833-45, 2010 (PubMed).

Kasahara, Goto, Enomoto, Tomono, Kiyono, Inagaki: "14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage." in: The EMBO journal, Vol. 29, Issue 16, pp. 2802-12, 2010 (PubMed).

Background publications Chow, Siu, Fung, Chan, Lau, Arooz, Ng, Yamashita, Poon: "DNA damage during the spindle-assembly checkpoint degrades CDC25A, inhibits cyclin-CDC2 complexes, and reverses cells to interphase." in: Molecular biology of the cell, Vol. 14, Issue 10, pp. 3989-4002, 2003 (PubMed).