PKB-GAMMA (AKT3) (S472) antibody

Antigen: PKB-GAMMA (AKT3)

Synonyms: PKBG, PRKBG, STK-2, PKB-GAMMA, RAC-gamma, RAC-PK-gamma, DKFZp434N0250

Epitope: S472

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389853

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: Phospho-AKT3-S472

Gene ID: 10000

Swiss-Prot: Q9Y243

Immunogen: This Phospho-AKT3-S472 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S472 of human AKT3.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, Protein kinase Akt-3, Protein kinase B, gamma, PKB gamma, STK-2, AKT3

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S472 of human AKT3.

Molecular Weight: 55775 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with hi

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab (ABIN389853) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Publications: Silva, Garvin: "Akt1 mediates purinergic-dependent NOS3 activation in thick ascending limbs." in: American journal of physiology. Renal physiology, Vol. 297, Issue 3, pp. F646-52, 2009 (PubMed).

Huang, Gonzalez, Toy et al.: "Blockade of CCN6 (WISP3) activates growth factor-independent survival and resistance to anoikis in human mammary epithelial cells." in: Cancer research, Vol. 70, Issue 8, pp. 3340-50, 2010 (PubMed).