CD14 Molecule (CD14) (N-Term), (AA 54-83) antibody

Details for Product No. ABIN390260
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Antigen
Epitope
N-Term, AA 54-83
(6), (6), (6), (6), (4), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(361), (68), (29), (28), (24), (20), (19), (14), (13), (8), (8), (8), (7), (7), (1), (1)
Host
Rabbit
(331), (59), (27), (10), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(55), (39), (36), (23), (12), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(292), (106), (96), (59), (48), (43), (41), (25), (22), (10), (10), (5), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN390260
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 AA from the N-terminal region of human CD14.
Clone RB14105
Isotype Ig
Specificity This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61~91 amino acids from the N-terminal region of human CD14.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name CD14
Background CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Molecular Weight 40076 DA
Gene ID 929
UniProt P08571
Research Area Apoptosis/Necrosis, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-CD14 Molecule (CD14) (N-Term), (AA 54-83) antibody Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (ABIN390260). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
anti-CD14 Molecule (CD14) (N-Term), (AA 54-83) antibody (2) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (ABIN390260), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-CD14 Molecule (CD14) (N-Term), (AA 54-83) antibody (3) CD14 Antibody (N-term) (ABIN390260) western blot analysis in A549 cell line lysates (35 µg/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
Background publications Goyert, Ferrero, Rettig et al.: "The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors." in: Science (New York, N.Y.), Vol. 239, Issue 4839, pp. 497-500, 1988 (PubMed).

Setoguchi, Nasu, Yoshida et al.: "Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones." in: Biochimica et biophysica acta, Vol. 1008, Issue 2, pp. 213-22, 1989 (PubMed).

Donati, Liljenberg, Padyukov et al.: "Local expression of interleukin-10 and mCD14 in relation to the -1087 IL-10 and -159 CD14 gene polymorphisms in chronic periodontitis." in: Journal of periodontology, Vol. 79, Issue 3, pp. 517-24, 2008 (PubMed).

Hosts (331), (59), (27), (10), (3)
Reactivities (361), (68), (29), (28), (24), (20), (19), (14), (13), (8), (8), (8), (7), (7), (1), (1)
Applications (292), (106), (96), (59), (48), (43), (41), (25), (22), (10), (10), (5), (3), (3), (2), (2), (1), (1), (1)
Conjugates (55), (39), (36), (23), (12), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (6), (6), (6), (4), (2), (2), (1), (1), (1), (1)
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