Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (C-Term), (AA 479-510) antibody

Details for Product No. ABIN391303
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Antigen
Epitope
C-Term, AA 479-510
(23), (21), (16), (15), (14), (12), (12), (11), (11), (8), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(209), (119), (112), (30), (28), (28), (28), (4), (3), (1), (1), (1)
Host
Rabbit
(196), (31), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB)
(170), (79), (68), (47), (41), (26), (21), (10), (9), (6), (3), (1), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN391303
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Immunogen This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-510 AA from the C-terminal region of human AMPK alpha 1.
Clone RB2520
Isotype Ig
Specificity This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487~517 amino acids from the C-terminal region of human AMPK alpha 1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name AMPK alpha 1
Background AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.
Synonyms: 5'-AMP-activated protein kinase, catalytic alpha-1 chain, AMPK alpha-1 chain
Molecular Weight 62793 DA
Gene ID 5562
UniProt Q13131
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (C-Term), (AA 479-510) antibody Western blot analysis of anti-AMPK alpha 1 C-term Pab (ABIN391303) in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (C-Term), (AA 479-510) antibody (2) AMPKalpha1 Antibody (C494) (ABIN391303) western blot analysis in SK-BR-3 cell line lysates (35 µg/lane).This demonstrates the AMPKalpha1 antibody detected the AMPKalpha1 protein (arrow).
Background publications Zhang, Ye, Wu et al.: "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." in: Genome research, Vol. 10, Issue 10, pp. 1546-60, 2000 (PubMed).

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