E2F1 antibody (E2F Transcription Factor 1) (AA 342-371)

Details for Product anti-E2F1 Antibody No. ABIN391858, Supplier: Log in to see
Antigen
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Alternatives
anti-Human E2F1 antibody for Cytometry by Time of Flight
Epitope
AA 342-371, His357
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Reactivity
Human
374
102
47
10
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Host
Rabbit
288
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21
1
Clonality (Clone)
Polyclonal ()
Conjugate
This E2F1 antibody is un-conjugated
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Application
Western Blotting (WB)
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167
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42
39
25
13
12
3
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1
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Options
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Immunogen This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 AA from human E2F1.
Clone RB7913
Isotype Ig
Specificity This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding H357 of human E2F1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Transcription factor E2F1, E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, E2F1, RBBP3
Molecular Weight 46920 DA
Gene ID 1869
UniProt Q01094
Research Area Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 342-371) (ABIN391858) Western blot analysis of E2F1 Antibody (H357) Pab (ABIN391858) pre-incubated without(...
Product cited in: Kim, Ju, Lim, Kang: "Maspin genetically and functionally associates with gastric cancer by regulating cell cycle progression." in: Carcinogenesis, Vol. 33, Issue 12, pp. 2344-50, 2012 (PubMed).

Background publications ODonnell, Wentzel, Zeller, Dang, Mendell: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005 (PubMed).

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005 (PubMed).

Joshi, Ordonez-Ercan, Dasgupta, Chellappan: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005 (PubMed).

Saberwal, Lucas, Janssen, Deobhakta, Hu, Galili, Raza, Mundle: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004 (PubMed).

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004 (PubMed).