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EPH Receptor B2 antibody (EPHB2) (AA 103-133)

Details for Product anti-EPHB2 Antibody No. ABIN391919, Supplier: Log in to see
Antigen
  • CAPB
  • Cek5
  • DRT
  • Drt
  • EK5
  • EPHT3
  • Erk
  • ERK
  • ETECK
  • Hek5
  • Nuk
  • PCBC
  • Prkm5
  • Qek5
  • RGD1564232
  • Sek3
  • Tyro5
Alternatives
anti-Human EPH Receptor B2 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 103-133, N-Term
33
19
15
14
10
8
8
7
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
142
34
4
3
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
80
39
19
14
Clonality (Clone)
Polyclonal ()
Conjugate
This EPH Receptor B2 antibody is un-conjugated
9
6
6
6
6
6
2
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
114
93
52
31
23
23
12
7
1
1
1
1
Supplier
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Immunogen This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-133 AA from the N-terminal region of human EphB2.
Clone RB01650
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EphB2 (EPHB2 Antibody Abstract)
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.

Synonyms: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK
Molecular Weight 117506 Da
Gene ID 2048
UniProt P29323
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology
Pathways RTK Signaling
Application Notes Suggested dilutions:
IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.47 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-EPH Receptor B2 antibody (EPHB2) (AA 103-133) (ABIN391919) Western blot analysis of hEPHB2-A118 (ABIN391919) in K562 cell line lysates (35 µg/la...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 antibody (EPHB2) (AA 103-133) (ABIN391919) EphB2 Antibody (N-term) (ABIN391919)immunohistochemistry analysis in formalin fixed a...
Product cited in: Xue, Huang, Wang, Dong, Zhou: "EphrinB2 and EphB4 expression in pterygia: new insights and preliminary results." in: Canadian journal of ophthalmology. Journal canadien d'ophtalmologie, Vol. 44, Issue 2, pp. 185-8, 2009 (PubMed).

Background publications Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999 (PubMed).

Ikegaki, Tang, Liu, Biegel, Allen, Yoshioka, Sulman, Brodeur, Pleasure: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996 (PubMed).

Iwase, Tanaka, Suzuki, Naito, Sugimura, Kino: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993 (PubMed).