V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) (Center) antibody

Antigen: V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS)

Synonyms: CTLO, HAMSV, HRAS1, K-RAS, N-RAS, RASH1, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1, c-H-ras, H-Ras, K-Ras, hras1, rash1, HRAS, ras, N-Ras, c-bas/has, zgc:110250, H-RAS, LOC100227892

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392183

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: HRAS (Center)

Gene ID: 3265

Swiss-Prot: P01112

Immunogen: This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108~138 amino acids from the Center region of human HRAS.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: GTPase HRas, Transforming protein p21, p21ras, H-Ras-1, c-H-ras, Ha-Ras, HRAS, HRAS1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human HRAS.

Molecular Weight: 21298 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-HRAS Antibody (Center) (ABIN392183) in Jurkat cell line lysates (35ug/lane). HRAS (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.