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NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9) (AA 846-877), (C-Term) antibody

Details for Product No. ABIN392654, Supplier: Log in to see
Antigen
  • C130021H08Rik
  • jck
  • MGC80499
  • MGC88903
  • Nek8
  • NEK9
  • nek12a
  • NERCC
  • nercc
  • NERCC1
  • nercc1
  • nphp9
Epitope
AA 846-877, C-Term
19
17
15
11
8
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
117
60
42
1
Host
Rabbit
111
14
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
80
43
28
25
23
22
10
7
6
1
1
Supplier
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Immunogen This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 846-877 AA from the C-terminal region of human NEK9.
Clone RB2989
Isotype Ig
Specificity This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 855~884 amino acids from the C-terminal region of human NEK9.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NEK9 (NEK9 Antibody Abstract)
Background NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2. NEK9 is activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents. This protein is not cell-cycle regulated but activity is higher in G0-arrested cells. NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP. Interaction is also noted with NEK6 and NEK7 family members. This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts. Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
Synonyms: Serine/threonine-protein kinase Nek9, NimA-related protein kinase 9, Nercc1 kinase, NIMA-related kinase 8
Molecular Weight 107149 DA
Gene ID 91754
UniProt Q8TD19
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9) (AA 846-877), (C-Term) antibody (ABIN392654) The anti-NEK9 Pab (ABIN392654) is used in Western blot to detect NEK9 in 293 cell lys...
Immunohistochemistry (IHC) image for anti-NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9) (AA 846-877), (C-Term) antibody (ABIN392654) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Tan, Lee: "Nek9, a novel FACT-associated protein, modulates interphase progression." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 9321-30, 2004 (PubMed).

Belham, Roig, Caldwell, Aoyama, Kemp, Comb, Avruch: "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases." in: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 34897-909, 2003 (PubMed).

Roig, Mikhailov, Belham, Avruch: "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression." in: Genes & development, Vol. 16, Issue 13, pp. 1640-58, 2002 (PubMed).

Holland, Milne, Garka, Johnson, Willis, Sims, Rauch, Bird, Virca: "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 16229-40, 2002 (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).