RFP antibody (Red Fluorescent Protein) (Biotin)

Details for Product anti-RFP Antibody No. ABIN401226, Supplier: Log in to see
Antigen
Reactivity
Discosoma
82
23
21
10
7
2
1
1
Host
Rabbit
69
37
14
9
1
Clonality
Polyclonal
Conjugate
This RFP antibody is conjugated to Biotin
6
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
106
71
47
39
32
29
7
5
3
3
1
1
1
1
1
1
1
Options
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Immunogen Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
Isotype IgG
Cross-Reactivity (Details) Ads. to Hu, Ms, Rt Serum Proteins
Characteristics Molar Ratio: 10-20 BAC molecules per IgG molecule
Purification Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
Gene ID 86600
UniProt Q9U6Y8
Application Notes Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used todetect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotinconjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP iswell suited to titrate RFP in solution. The detection antibody conjugated to biotin issubsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugatedpolyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cellexpression systems and can detect RFP containing inserts. Significant amplification ofsignal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to thefluorescence of RFP alone. For immunoblotting use either alkaline phosphatase orperoxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins onwestern blots. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-RFP antibody (Red Fluorescent Protein)  (Biotin) (ABIN401226) anti-Red Fluorescent Protein (RFP) antibody (Biotin)
Background publications Gross, Baird, Hoffman, Baldridge, Tsien: "The structure of the chromophore within DsRed, a red fluorescent protein from coral." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 22, pp. 11990-5, 2000 (PubMed).