RFP antibody (Red Fluorescent Protein) (HRP)

Details for Product anti-RFP Antibody No. ABIN401228, Supplier: Log in to see
Antigen
Reactivity
Discosoma
82
50
36
10
7
2
1
1
Host
Rabbit
96
38
14
9
1
Clonality
Polyclonal
Conjugate
This RFP antibody is conjugated to HRP
9
6
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
133
72
61
53
33
29
7
5
3
3
1
1
1
1
1
1
1
Options
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Supplier Product No.
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Immunogen Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
Isotype IgG
Cross-Reactivity (Details) Ads. to Hu, Ms, Rt Serum Proteins
Purification Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Background Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
Gene ID 86600
UniProt Q9U6Y8
Application Notes ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 1,000 - 1: 5,000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (Immunoglobulin and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Do Not Add Sodium Azide
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. Following restoration product can be stored undiluted at 2-8 °C for up to one month or (in aliquots) at-20 °C or below. Centrifuge product if not completely clear after standing at room temperature.
Supplier Images
 image for anti-Red Fluorescent Protein (RFP) antibody (HRP) (ABIN401228) anti-Red Fluorescent Protein (RFP) antibody (HRP)
Background publications Gross, Baird, Hoffman, Baldridge, Tsien: "The structure of the chromophore within DsRed, a red fluorescent protein from coral." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 22, pp. 11990-5, 2000 (PubMed).

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