Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
Cross-Reactivity (Details)
Ads. to Hu, Ms, Rt Serum Proteins
Purification
Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Immunogen
Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 1,000 - 1: 5,000. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 0.1 mL of deionized water (or equivalent).
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (Immunoglobulin and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative
Preservative
Gentamicin sulfate
Precaution of Use
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Do Not Add Sodium Azide
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. Following restoration product can be stored undiluted at 2-8 °C for up to one month or (in aliquots) at-20 °C or below. Centrifuge product if not completely clear after standing at room temperature.
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag