This affinity antibody is directed against Human TGFbeta1 protein. Cross-reactivity is expected with TGFbeta1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.
Cross-Reactivity (Details)
Species reactivity (expected):A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100 % homology for the immunogen sequence. Species reactivity (tested):Human.
Purification
Affinity Chromatography
Immunogen
Synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length)
TGFB1
Reactivity: Human, Mouse, Rat
IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/1,000-1/5,000. Western blot: 1/100-1/500 (Expect a band approximately 12.5 kDa in size corresponding toreduced mature TGFB1 in the appropriate cell lysate or extract). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or for longer (in aliquots) at-20 °C or below for longer.
TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, including three mammalian TGFbeta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25 kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule, the amino acid sequence between human and mouse differs by only one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues, such as placenta and kidneys.Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1