SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody

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Antigen
Synonyms madh3, smad3, madh3a, wu:fa99e03, XSmad3, XenMLP, Xmad3, madh3-A, HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, AU022421, Madh3, Smad 3, mad3
Epitope
AA 417-425, C-Term
(50), (32), (29), (26), (23), (21), (12), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(308), (196), (189), (65), (52), (41), (15), (12), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(286), (34)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(211), (151), (93), (84), (70), (37), (19), (8), (5), (4), (3), (3), (2), (2), (1)
Pubmed 3 references available
Catalog no. ABIN401333
Quantity 0.1 mg
Price
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Immunogen Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein
Isotype IgG
Specificity This antibody is directed against Smad3 protein. ELISA and western blot show equivalent reactivity against phosphorylated and non- phosphorylated Human Smad3.
Purification Affinity Chromatography
Alternative Name SMAD3
Background Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (RSmads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-beta pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-beta- mediated response.
Alternate names: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3
Gene ID 4088
NCBI Accession NP_001138574.1
UniProt P84022
Research Area Signaling, Transcription Factors
Application Notes ELISA: 1/10,000 - 1/40,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/500 - 1/3,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.17 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody (2) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (C-Term) antibody (Image 2)
Background publications Zhang, Feng, We et al.: "Receptor-associated Mad homologues synergize as effectors of the TGF-beta response." in: Nature, Vol. 383, Issue 6596, pp. 168-72, 1996 (PubMed).

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).

Derynck, Zhang: "Smad-dependent and Smad-independent pathways in TGF-beta family signalling." in: Nature, Vol. 425, Issue 6958, pp. 577-84, 2003 (PubMed).

Hosts (286), (34)
Reactivities (308), (196), (189), (65), (52), (41), (15), (12), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Applications (211), (151), (93), (84), (70), (37), (19), (8), (5), (4), (3), (3), (2), (2), (1)
Conjugates (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2)
Epitopes (50), (32), (29), (26), (23), (21), (12), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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