Cyclin E2 antibody (C-Term)

Catalog No. ABIN401374

Product Details anti-Cyclin E2 Antibody

Target: Cyclin E2 (CCNE2)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against Cyclin E2 pr

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/20,000 - 1/85,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Cyclin E2

Target: Cyclin E2 (CCNE2)

Alternative Name: Cyclin E2 (CCNE2 Products)

Synonyms: ccne2 antibody, MGC53684 antibody, MGC132360 antibody, cb165 antibody, sb:cb165 antibody, zgc:86724 antibody, CCNE2 antibody, cyce2 antibody, CYCE2 antibody, cyclin E2 antibody, cyclin E2 L homeolog antibody, cyclin E1 L homeolog antibody, CCNE2 antibody, ccne2.L antibody, ccne2 antibody, ccne1.L antibody, Ccne2 antibody

Background: Cyclin E was first identified by its ability to rescue growth of yeast deficient in G1 Cyclins, indicating a role in G1 or G1/S transitions. Over-expression of Cyclin E has been observed in a variety of human tumors. Multiple isoforms of Cyclin E are expressed in tumors but not in normal tissues, suggesting a post-transcriptional regulation of Cyclin E. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27Kip1 and p21Cip1. The catalytic activity associated with Cyclin E2 complexes is cell cycle regulated and peaks at the G1/S transition. Unlike Cyclin E1, which is expressed in most proliferating normal and tumor cells, Cyclin E2 levels were low to undetectable in nontransformed cells and increased significantly in tumor-derived cells.Synonyms: CCNE2, Cyclin-E2, G1/S-specific cyclin-E2

Gene ID: 12448

NCBI Accession: NP_001032211

UniProt: Q9Z238

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases