Cofilin1/2 (CFL1/2) (pTyr88) antibody

Details for Product No. ABIN401588
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Synonyms CFL
(5), (2)
Human, Mouse (Murine), Rat (Rattus)
(9), (9), (5)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(8), (4), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN401588
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Cofilin-1/Cofilin-2 around the phosphorylation site of Tyrosine 88 (A-T-Yp-E-T).
Specificity Antibody detects endogenous levels of Cofilin-1/Cofilin-2 only when phosphorylated at Tyrosine 88.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Cofilin-1+2
Background Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their ‘pointed’ (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.
Alternate names: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18
Gene ID 1072
NCBI Accession NP_005498
UniProt P23528
Application Notes Immunohistochemistry on Paraffin Sections: 1/50~1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20°C.
Expiry Date 12 months
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anti-Cofilin1/2 (CFL1/2) (pTyr88) antibody anti-Cofilin1/2 (CFL1/2) (pTyr88) antibody
Background publications Arber, Barbayannis, Hanser et al.: "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase." in: Nature, Vol. 393, Issue 6687, pp. 805-9, 1998 (PubMed).

Yang, Higuchi, Ohashi et al.: "Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization." in: Nature, Vol. 393, Issue 6687, pp. 809-12, 1998 (PubMed).

Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

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