MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (Ser2481)

Details for Product anti-FRAP1 Antibody No. ABIN401643, Supplier: Log in to see
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Alternatives
anti-Human MTOR antibody for Immunofluorescence (fixed cells)
Epitope
Ser2481
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Options
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Immunogen Synthesized non-phosphopeptide derived from human mTOR around the phosphorylation site of serine 2481 (I-H-SP-F-I).
Specificity MTOR Antibody detects endogenous levels of total mTOR protein.
Purification Affinity chromatography
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background MTOR, or FKBP12 rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 289- kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events
Application Notes Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (Ser2481) (ABIN401643) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (Ser2481) antibody
Background publications Dennis, Jaeschke, Saitoh, Fowler, Kozma, Thomas: "Mammalian TOR: a homeostatic ATP sensor." in: Science (New York, N.Y.), Vol. 294, Issue 5544, pp. 1102-5, 2001 (PubMed).

Stull, Hyun, Sharetzsky, Wooten, McCauley, Smith: "Production and oxidation of indole by Haemophilus influenzae." in: The Journal of biological chemistry, Vol. 270, Issue 1, pp. 5-8, 1995 (PubMed).

Brown, Albers, Shin, Ichikawa, Keith, Lane, Schreiber: "A mammalian protein targeted by G1-arresting rapamycin-receptor complex." in: Nature, Vol. 369, Issue 6483, pp. 756-8, 1994 (PubMed).

Carraway, Cantley: "A neu acquaintance for erbB3 and erbB4: a role for receptor heterodimerization in growth signaling." in: Cell, Vol. 78, Issue 1, pp. 5-8, 1994 (PubMed).