Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (Middle Region) antibody

Details for Product No. ABIN405954, Supplier: Login to see New
New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms succinate dehydrogenase 2-3, CG3283, Dmel\\CG3283, Ip, SDH, SDH-IP, SDH-Ip, SDHb, sdhB, CWS2, IP, PGL4, SDH1, SDH2, SDHIP, 0710008N11Rik, fj46d06, wu:fj46d06
Epitope
Middle Region
(27), (15), (13), (12), (6), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(86), (36), (22), (3), (1), (1), (1)
Host
Rabbit
(69), (19), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(68), (29), (15), (11), (11), (10), (9), (2), (1), (1)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 50 μg
Options
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Synthetic peptide directed towards the middle region of human SDHB
Blocking Peptide Blocking peptide for this product available: Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (Middle Region) peptide (ABIN983488)
Sequence YRWMIDSRDD FTEERLAKLQ DPFSLYRCHT IMNCTRTCPK GLNPGKAIAE
Predicted Reactivity Bovine : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %
Characteristics This is a rabbit polyclonal antibody against SDHB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SDHB (SDHB Antibody Abstract)
Background Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.
Molecular Weight 31 kDa
Gene ID 6390
NCBI Accession NM_003000, NP_002991
UniProt P21912
Research Area Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 280 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB) (Middle Region) antibody (ABIN405954) WB Suggested Anti-SDHB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Product cited in: Qattan, Radulovic, Crawford et al.: "Spatial distribution of cellular function: the partitioning of proteins between mitochondria and the nucleus in MCF7 breast cancer cells." in: Journal of proteome research, Vol. 11, Issue 12, pp. 6080-101, 2012 (PubMed).

Background publications Cascón, Landa, López-Jiménez et al.: "Molecular characterisation of a common SDHB deletion in paraganglioma patients." in: Journal of medical genetics, Vol. 45, Issue 4, pp. 233-8, 2008 (PubMed).

Catalog No. ABIN405954
$289.00
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)