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Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (N-Term) antibody

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Antigen
Synonyms UBE1C, hUBA3, A830034N06Rik, AI256736, AI848246, AW546539, Ube1c, ube1c, wu:fb75e04, wu:fc37b11, zgc:55528
Epitope
N-Term
(20), (3), (2), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(30), (9), (4), (1), (1), (1), (1), (1)
Host
Rabbit
(30)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(28), (17), (17), (4), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human UBA3
Blocking Peptide Blocking peptide for this product available: ABIN985798
Sequence EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV IGAGGLGCEL
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against UBA3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name UBA3 (UBA3 Antibody Abstract)
Background UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Weight 52 kDa
Gene ID 9039
NCBI Accession NP_003959, NM_003968
UniProt Q8TBC4
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 463 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (N-Term) antibody (ABIN406124) WB Suggested Anti-UBA3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (N-Term) antibody (ABIN406124) Sample Type: 1. Human NT-2 cells (60ug)
2. mouse brain extracts (80ug)
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Background publications Li, Santockyte, Shen et al.: "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers." in: Archives of biochemistry and biophysics, Vol. 453, Issue 1, pp. 70-4, 2006 (PubMed).

Catalog No. ABIN406124
$289.00
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