CRYAB antibody

Catalog No. ABIN453950

Product Details anti-CRYAB Antibody

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects a band at ~20 kDa corresponding to Alpha B Crystallin. Does not cross react with Alpha A Crystallin, Beta-L Crystallin, Beta-H Crystallin, Gamma Crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine.

Characteristics: Synonyms: Alpha(B)-crystallin, CRYAB, CRYA2, Heat shock protein beta-5, Renal carcinoma antigenNY-REN-27, HspB5, Rosenthal fiber component

Purification: Protein G Affinity Chromatography

Immunogen: Native alpha B crystallin

Clone: 1A7-D5

Isotype: IgG1

Application Details

Application Notes: Western blot (10): 1/2000. ELISA.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: alpha-Crystallin B Chain (CRYAB Products)

Synonyms: CMD1II antibody, CRYA2 antibody, CTPP2 antibody, CTRCT16 antibody, HSPB5 antibody, MFM2 antibody, Crya-2 antibody, Crya2 antibody, HspB5 antibody, AACRYA antibody, CRYAB antibody, cryab antibody, cryab2 antibody, wu:fe37f08 antibody, zgc:91937 antibody, crystallin alpha B antibody, crystallin, alpha B antibody, hypothetical protein antibody, crystallin, alpha B, a antibody, crystallin, alpha B, b antibody, CRYAB antibody, Cryab antibody, ZK1128.7 antibody, cryaba antibody, cryabb antibody

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alphacrystallins possess structural and functional imilarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).Synonyms: Alpha(B)-crystallin, CRYA2, CRYAB, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Molecular Weight: approx. 21 kDa (Predicted)

Gene ID: 1410

UniProt: P02511