PSMB5 antibody

Catalog No. ABIN462514

Product Details anti-PSMB5 Antibody

Target: PSMB5 (Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Pig, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This PSMB5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PSMB5.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Platypus, Xenopus, Catfish, Smelt, Stickleback, Pufferfish, Zebrafish, Sea anemone (100%) Salmon, Seq squirt, Mosquito, Water flea, Capsaspora (92%) Chicken, Trout, Ant, Beetle, Trichoplax, Tick, Tunicate (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human PSMB5 / MB1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Platypus, Xenopus, Catfish, Smelt, Stickleback, Pufferfish, Zebrafish, Sea anemone (100%), Salmon, Seq squirt, Mosquito, Water flea, Capsaspora (92%), Chicken, Trout, Ant, Beetle, Trichoplax, Tick, Tunicate (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PSMB5

Target: PSMB5 (Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5))

Alternative Name: PSMB5 / MB1 (PSMB5 Products)

Synonyms: LMPX antibody, MB1 antibody, X antibody, etID309919.2 antibody, fb59c07 antibody, si:zc14a17.13 antibody, wu:fb59c07 antibody, x antibody, zgc:86820 antibody, proteasome subunit beta 5 antibody, proteasome (prosome, macropain) subunit, beta type 5 antibody, proteasome subunit beta 5 L homeolog antibody, proteasome (prosome, macropain) subunit, beta type, 5 antibody, PSMB5 antibody, psmb5 antibody, Psmb5 antibody, psmb5.L antibody

Background: Name/Gene ID: PSMB5
Subfamily: Threonine T1
Family: Protease

Synonyms: PSMB5, Proteasome chain 6, Proteasome subunit MB1, Proteasome subunit X, Proteasome catalytic subunit 3, Macropain epsilon chain, MB1, LMPX, Proteasome beta 5 subunit, Proteasome epsilon chain, Proteasome subunit beta type-5

Gene ID: 5693

UniProt: P28074

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA