E2F1 antibody (AA 107-156)
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- Target See all E2F1 Antibodies
- E2F1 (E2F Transcription Factor 1 (E2F1))
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Binding Specificity
- AA 107-156
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Reactivity
- Human, Mouse, Rat, Monkey, Dog, Guinea Pig, Horse, Rabbit, Bat, Pig, Chicken
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This E2F1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Human E2F1
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Xenopus (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa107-156 of human E2F1 (Q01094, NP_005216). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Lizard (100%), Xenopus (85%), Monkey, Panda, Bovine, Turkey, Platypus (84%), Zebra finch (83%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product E2F1 Primary Antibody
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- Application Notes
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- E2F1 (E2F Transcription Factor 1 (E2F1))
- Alternative Name
- E2F1 (E2F1 Products)
- Synonyms
- CG6376 antibody, DRTF1/E2F antibody, DmE2F-1 antibody, Dmel\\CG6376 antibody, Dp antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E(var)3-95E antibody, E(var)93E antibody, E2F antibody, E2F-1 antibody, E2F1 antibody, E2f1 antibody, Evar(3)164 antibody, dE2F antibody, dE2F1 antibody, dE2f antibody, dE2f1 antibody, de2f1 antibody, def21 antibody, drosE2F1 antibody, e2f antibody, e2f1 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, mKIAA4009 antibody, xE2F antibody, ATE2FB antibody, E2F TRANSCRIPTION FACTOR-1 E2F1 antibody, E2F transcription factor 1 antibody, E2FB antibody, E2FB TRANSCRIPTION FACTOR antibody, E2F transcription factor 1 antibody, E2F transcription factor 1 L homeolog antibody, E2f1 antibody, E2F1 antibody, e2f1.L antibody
- Background
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Name/Gene ID: E2F1
Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3 - Gene ID
- 1869
- NCBI Accession
- NP_005216
- UniProt
- Q01094
- Pathways
- p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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