Leptin antibody (Biotin)
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- Target See all Leptin (LEP) Antibodies
- Leptin (LEP)
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Leptin antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Specificity
- Mouse Leptin
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Murine Leptin.
Type of Immunogen: Cell extract - Top Product
- Discover our top product LEP Primary Antibody
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- Application Notes
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mLeptin by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Leptin (LS-C104760) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- Leptin (LEP)
- Alternative Name
- Leptin (LEP Products)
- Synonyms
- ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
- Background
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Name/Gene ID: LEP
Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB - Gene ID
- 3952
- UniProt
- P41159
- Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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