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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)

Synonyms
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ... show more
Epitope
Alternatives

pThr450

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Catalog no. ABIN481865
Quantity 50µg  (1 mg/ml)
Price 150.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name Akt1 (Phospho-Thr450)
Gene ID 207 (NCBI Gene Symbol: AKT1)
Swiss-Prot P31749
Immunogen The antiserum was produced against synthesized peptide derived from human Akt1 around the phosphorylation site of Thr450.
Format Liquid
Isotype IgG  (Matching secondary antibodies)
Description Synonyms: AKT, AKT1 kinase, C-AKT, kinase Akt1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-alpha serine/threonine kinase, RAC-PK-alpha
Specificity Akt1 (Phospho-Thr450) Antibody detects endogenous levels of Akt1 only when phosphorylated at threonine450.
Molecular Weight 55 kDa
Comments

OMIM-Number: 164730, Unigene-Number: Hs.525622

Synonyms AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details

Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Concentration 1 mg/ml
Purity Greater 95%
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C/1 year
Restrictions For Research Use only

Alternatives

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts Rabbit (186), Mouse (61), Sheep (3), Chicken (1)
Reactivities Human (231), Rat (Rattus) (117), Mouse (Murine) (114), Dog (Canine) (10), Chicken (8), Monkey (6), Chimpanzee (2), Cow (Bovine) (2), Sheep (Ovine) (1)
Applications Western Blotting (WB) (207), Immunohistochemistry (IHC) (78), Enzyme Immunoassay (EIA) (63), Immunofluorescence (IF) (62), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), ELISA (45), Immunoprecipitation (IP) (17), Flow Cytometry (FACS) (11), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Dot Blot (Dot) (9), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
Conjugates Biotin (2), APC (1), CF (1), PE (1)
Epitopes pSer473 (22), pThr308 (14), pThr450 (4), pTyr315/316/312 (4), S129 (3), C-Term (2), N-Term (2), S246 (2), pS124 (2), pS473 (2), pThr308/309 (2), pTyr326 (2), pY326 (2), N-Term G37 (1), S124 (1), S473 (1), pSer124 (1), pSer129 (1), pT308 (1)