RASGRF1 antibody (pSer916)

Catalog No. ABIN482083

This Rabbit Polyclonal antibody specifically detects RASGRF1 in IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for RASGRF1 antibody (pSer916) (ABIN482083)

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-RASGRF1 Antibody

Binding Specificity: AA 882-931, pSer916

Specificity:

Ras-GRF1 (Phospho-Ser916) Antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at Ser916.

PhosphorylationM:S916 R:S898

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Mm.44561 (NCBI Gene Symbol: RASGRF1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: Ras-GRF1

Background:

Synonyms: CDC25, GNRP, GRF1, Guanine nucleotide releasing protein, P140 Ras-GRF, RASGRF1, Ras-specific nucleotide exchange factor CDC25

NCBI Gene Symbol: RASGRF1

Molecular Weight: 145 kDa

Gene ID: 19417

UniProt: P27671

Pathways: RTK Signaling, Cell Division Cycle, M Phase