GRK2 antibody (pSer685)

Catalog No. ABIN482730

The Rabbit Polyclonal anti-GRK2 antibody (ABIN482730) specifically detects GRK2 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GRK2 antibody (pSer685) (ABIN482730)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GRK2 Antibody

Binding Specificity: AA 640-689, pSer685

Specificity:

GRK2 (Phospho-Ser685) Antibody detects endogenous levels of GRK2 only when phosphorylated at Ser685.

PhosphorylationH:S685 M:S685 R:S685

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser685.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.83636 (NCBI Gene Symbol: ADRBK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2

Background:

Synonyms: ADRBK1, ARBK1, BARK, BARK1, beta-adrenergic receptor kinase 1, beta-ARK-1, G protein receptor kinase 2, G- protein coupled receptor kinase 2, kinase GRK2

NCBI Gene Symbol: ADRBK1

Molecular Weight: 79 kDa

Gene ID: 156

OMIM: 109635

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling