Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.5-1 μg/mL. Approx 100 kDa band observed in lysates of the cell linesHEK293 and NIH3T3 (calculated MW of 93.0 kDa according to NP_001034200.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
TRIM71
(Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))
TRIM71 may be involved in controlling the timing of organ formation during development.Synonyms: LIN41, Protein lin-41 homolog, Tripartite motif-containing protein 71