GRK2 antibody

Catalog No. ABIN499029

The Rabbit Polyclonal anti-GRK2 antibody (ABIN499029) specifically detects GRK2 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GRK2 antibody (ABIN499029)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRK2 Antibody

Specificity: This antibody detects endogenous levels of GRK 2 protein. (region surrounding Val678)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: beta-ARK-1 / ADRBK1

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1, two forms of β-adrenergic receptor kinase, GRK 2 (βARK, βARK1) and GRK 3 (βARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.Synonyms: BARK, BARK1, Beta-adrenergic receptor kinase 1, G-protein coupled receptor kinase 2, GRK2

Molecular Weight: approx. 80 kDa

Gene ID: 156

NCBI Accession: NP_001610

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling