MDFI antibody

Catalog No. ABIN499093

Product Details anti-MDFI Antibody

Target: MDFI (MyoD Family Inhibitor (MDFI))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDFI antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of MDFI protein. (region surrounding Pro134)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for MDFI

Target: MDFI (MyoD Family Inhibitor (MDFI))

Alternative Name: MDFI (MDFI Products)

Synonyms: MDFI antibody, I-MF antibody, I-mfa antibody, I-mf antibody, RGD1560271 antibody, MyoD family inhibitor antibody, MDFI antibody, Mdfi antibody

Background: MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.Synonyms: MyoD family inhibitor

Molecular Weight: approx. 25 kDa

Gene ID: 4188

NCBI Accession: NP_005577

UniProt: Q99750

Pathways: Maintenance of Protein Location