V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

Details for Product No. ABIN499261
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Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(936), (554), (523), (165), (155), (116), (95), (24), (18), (4), (3), (1), (1), (1), (1)
Host
Rabbit
(799), (144), (10), (5), (3), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(34), (28), (27), (22), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(718), (430), (297), (149), (142), (133), (116), (65), (58), (41), (11), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN499261
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Immunogen Human Akt1 / Protein kinase B / PKB Peptide
Isotype IgG
Specificity Akt1 antibody was raised against a 16 amino acid peptide from near the amino-terminus of human Akt1.
Purification Affinity chromatography purified via peptide column
Alternative Name AKT1 / PKB
Background Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt (1), is part of the phosphatidyl 3’-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival (2). Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2 (3). The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad (4,5). Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors (reviewed in 6).
Alternate names: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes ELISA. Western Blot: Akt1 antibody can be used for the detection of Akt1 at 1 μg/ml. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Bellacosa, Testa, Staal et al.: "A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region." in: Science (New York, N.Y.), Vol. 254, Issue 5029, pp. 274-7, 1991 (PubMed).

Alessi, Andjelkovic, Caudwell et al.: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997 (PubMed).

Navé, Ouwens, Withers et al.: "Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation." in: The Biochemical journal, Vol. 344 Pt 2, pp. 427-31, 2000 (PubMed).

Song, Ouyang, Bao: "The activation of Akt/PKB signaling pathway and cell survival." in: Journal of cellular and molecular medicine, Vol. 9, Issue 1, pp. 59-71, 2005 (PubMed).

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