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MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term)

Details for Product anti-MAP3K5 Antibody No. ABIN499345, Supplier: Log in to see
Antigen
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
Alternatives
anti-Human MAP3K5 antibody for Western Blotting
Epitope
C-Term
53
43
39
23
22
19
17
15
13
13
13
9
8
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
360
139
79
4
1
Host
Rabbit
346
22
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
6
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
279
141
125
56
50
36
20
10
6
6
6
2
1
1
1
Supplier
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Immunogen Human ASK1 Peptide
Isotype IgG
Specificity ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.
Purification Affinity chromatography purified via peptide column
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling
Application Notes ELISA. Western Blot: ASK1 antibody can be used for detection of ASK1 at 1: 500 to 1: 1000 dilution. A 155 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term) (ABIN499345) Immunocytochemistry of ASK1 in A431 cells with AP30085PU-N ASK1 antibody at 10 μg/ml.
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term) (ABIN499345) Immunofluorescence of ASK1 in A431 cells with AP30085PU-N ASK1 antibody at 20 μg/ml.
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term) (ABIN499345) Western blot analysis of ASK1 in SW1353 whole cell lysate with AP30085PU-N ASK1 antib...
Background publications Tobiume, Inage, Takeda et al.: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997 (PubMed).

Ichijo, Nishida, Irie et al.: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997 (PubMed).

Wang, Diener, Jannuzzi et al.: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997 (PubMed).