Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (C-Term) antibody

Details for Product No. ABIN499573
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Antigen
Synonyms casp8-A, xCaspase-8, casp8, CASP8, CASP-8, FLICE, MACH, Mch5, ALPS2B, CAP4, Casp-8, MCH5, caspase-8, zgc:92075
Epitope
C-Term
(28), (19), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(217), (72), (57), (25), (13), (12), (12), (9), (5), (2), (1), (1)
Host
Rabbit
(185), (54), (6), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(214), (86), (50), (47), (35), (26), (16), (13), (13), (10), (3), (2), (2), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN499573
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Immunogen Virus Casp-8 / FLICE / MACH / Mch5 (C-Terminus) Peptide
Isotype IgG
Specificity Caspase-8 antibody was raised against a 16 amino acid peptide from near the carboxy- terminus of human Caspase-8 isoform A.
Purification Affinity chromatography purified via peptide column
Alternative Name Caspase-8
Background Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex (4). The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain, subsequent oligomerization promotes procaspase-8 autoactivation (reviewed in 5). FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis (6).
Alternate names: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD- homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog
Gene ID 841
NCBI Accession NP_001219
UniProt Q14790
Research Area Apoptosis/Necrosis
Application Notes ELISA. Western Blot: Caspase-8 antibody can be used for the detection of caspase-8 at 0.5 μg/ml. Depending on cell lines or tissues used, either full-length or other cleavage products maybe observed. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).

Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999 (PubMed).

Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004 (PubMed).

Zhivotovsky, Orrenius: "Caspase-2 function in response to DNA damage." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 859-67, 2005 (PubMed).

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