Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) (Center) antibody

Details for Product No. ABIN500032
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Antigen
Synonyms LOC100219853, CD49D, IA4
Epitope
Center
(15), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(152), (121), (35), (6), (4), (4), (1), (1), (1), (1), (1)
Host
Rabbit
(105), (105), (44)
Clonality
Polyclonal
Conjugate
Un-conjugated
(45), (25), (25), (11), (2), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(199), (70), (46), (44), (32), (24), (24), (20), (17), (13), (5), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN500032
401.50 $
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Immunogen Integrin alpha 4 antibody was raised against a 15 amino acid peptide from near the center of human Integrin alpha 4. (AP30436CP-N)
Isotype IgG
Specificity This antibody reacts to Integrin alpha 4.
Purification Affinity chromatography purified via peptide column
Alternative Name CD49d / ITGA4
Background The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha 4 recombinant protein fragment and thus will recognize it.
Alternate names: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Gene ID 3676
UniProt P13612
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell/Tissue Markers, Extracellular Matrix, Signaling
Application Notes ELISA. Western Blot: 0.5 – 1 μg/ml. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Pulido, Elices, Campanero et al.: "Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10241-5, 1991 (PubMed).

Kamata, Puzon, Takada: "Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29)" in: The Biochemical journal, Vol. 305 ( Pt 3), pp. 945-51, 1995 (PubMed).

Berlin, Berg, Briskin et al.: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

Wegener, Campbell: "Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions (review)." in: Molecular membrane biology, Vol. 25, Issue 5, pp. 376-87, 2008 (PubMed).

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